To determine whether S4KO alleviates renal fibrosis by upregulating the acetylation of U2AF2 in mouse kidney, we crossed Cdh16-cre/ERT2×U2af2flox/flox (U2af2-/-) with Sirt4-/- mice to generate DKO mice and then performed UUO surgery. The gene discussed is CDH16; the disease is renal fibrosis.