The deafness-associated mutation is located in TM 4 (Figure 1—figure supplement 1f; equivalent of M522 in TMEM16F, I547 in TMEM16A, A439 in OSCA1.2, and A476 in TMEM63A) and results in constitutive PS exposure when expressed in the hair cell membranes of both heterozygous and homozygous mice (Ballesteros and Swartz, 2022). This evidence concerns the gene ANO6 and deafness.