The results showed that the total CD8+ T cell infiltration in the tumor immune microenvironment was significantly increased in the knockdown of PLCG2 group (sh-PLCG2), with the significant increase of GZMB+CD8+ T cells, PRF1+CD8+ T cells, TNF-α+CD8+ T cells and IFN-γ+CD8+ T cells, and the significant decrease of PD-1+CD8+ T cells, compared to the control group (shnc-PLCG2) (Figure 9F). The gene discussed is PRF1; the disease is neoplasm.