To confirm whether the link between β-catenin localisation and SIRT1 expression and deacetylase activity also takes place in human CRC, we next analysed β-catenin subcellular distribution and SIRT1 level and activity in tissue microarrays (TMA) containing samples (n = 107) from colorectal primary tumours of stage II patients and liver metastases of stage IV patients. The gene discussed is SIRT1; the disease is colorectal carcinoma.