HES4 and colon adenocarcinoma: Figure 6A shows the immune infiltration profile of various cells within the TME of COAD, while Fig. 6B shows the correlation between immune cell scores. According to the immune infiltration score, we performed Spearman’s correlation analysis between the expression levels of HES4, TINNC 1, RNF208, ADAM12, DRD4, POLR2L, RHOD, WDR24, ZNF771, and PRMT1 and immune cells. We found that the expression of several mRNAs was correlated with immune cell infiltration (Figs. 6C–6L).