The ace gene was commonly detected in HBM and clinical isolates except Symbioflor 1, which contributes to E. faecalis colonization in the heart valves and may cause endocarditis (Silva, Montalvao & Bonafe, 2017).The ebp operon-encoded pili (endocarditis-associated pili) also contribute to biofilm formation and binding of thrombocytes, collagen, and fibrinogen (Krawczyk et al., 2020). The gene discussed is ACE; the disease is endocarditis.