Female MSEW mice fed a HFD show exacerbated obesity and aldosterone production compared with diet-matched control mice, while chronic treatment in adulthood with spironolactone, a mineralocorticoid receptor antagonist, resulted in loss of fat mass coupled with increased glycerol efflux and decreased adipocyte size in MSEW females, but not in HFD controls (24). Here, NR3C2 is linked to obesity due to melanocortin 4 receptor deficiency.