CD4 and gastric cancer: To evaluate the presence and maturity of TLS, the general composition of TLSs in GC patients was investigated using conventional immunohistochemistry in serial sections stained for CD8+ T cells (Figure 1E), CD19+ B cells (Figure 1F), CD45+ lymphocytes (Figure 1G), CD11c+ dendritic cells (Figure 1H), CD4+ T cells (Figure 1I), as well as CD68+ macrophages (Figure 1J).