CTLA-4 and TIGIT were expressed by an approximately similar proportion of Vγ9Vδ2 T-cells in cancer patient and healthy donor PBMC and were in both cases upregulated upon expansion, though the proportion of Vγ9Vδ2 T-cells expressing CTLA-4 appeared to be more consistently increased upon activation and expansion in cancer patient PBMC. Here, CTLA4 is linked to cancer.