To identify master CRC TFs orchestrating the epigenetic reprogramming in GBC, we utilized the “Coltron” algorithm to analyze the SE profile of GBC and control samples (Figure S2E,F, Supporting Information).[20, 32] Considering sample heterogeneity, we defined the CRC TFs as those presented in at least 50% of tumor samples’ or in both CC samples’ predictions and identified a total of 43 CRC TF candidates. The gene discussed is TF; the disease is cholangiocarcinoma.