To further assess the consistency of Piezo1 impact on effector T-cell differentiation and persistence in vivo, we revisited the colitis model and employed an adoptive transfer model of chronic intestinal inflammation that requires induction of transferred naive CD4+ T-cell migration, activation, and effector function for pathogenesis.46,47 Naive CD4+ T cells (CD4+CD45RBhigh) from Piezo1WT or Piezo1cKO were injected into Rag1−/− mice to induce intestinal inflammation. Here, CD4 is linked to inflammation.