Our previous work demonstrated that RNAi-mediated Notch1 inhibition promoted an inflamed TME by reducing immunosuppressive cytokines and factors (e.g., IL10, TGFβ, ARG1), immunosuppressive cells (MDSCs and Tregs); and by increasing IFNγ and CD8+ T cells in the melanoma TME. Here, CD8A is linked to melanoma.