To thoroughly test the anti-tumor activity of anti-N1, the mouse syngeneic melanoma lines YUMM2.1, which respond to ICI, and YUMM1.7, which is instead resistant [25, 26], were inoculated subcutaneously in C57Bl/6 mice, which retain an intact immune system, allowing analysis of Notch1 blockade on the TME. Here, NOTCH1 is linked to melanoma.