A three-day treatment with anti-N1 induced 75%, 80% and 55% cell death in the K457 (human) and the YUMM1.7 and YUMM2.1 (mouse) melanoma lines, respectively (Fig. 3A-D), but did not affect either human or mouse melanocytes, which express low or undetectable Notch1 levels compared to melanoma cells. This evidence concerns the gene NOTCH1 and melanoma.