CD4 and neoplasm: Second, combination therapy (cryoablation + anti-PD-1 antibody) leads to a conversion of the distant tumor TME from immunosuppressive to immunostimulatory, favoring the infiltration of CD8+T cells, CD4+T cells, DCs and M1-like TAMs, enhancing multiple aspects of the antitumor immune response while simultaneously reducing immunosuppressive populations such as M2-like TAMs and MDSCs.