Further research revealed that O-GlcNAcylation of ryanodine receptor 1 (RYR1) interferes with NEK10-mediated phosphorylation, increasing ubiquitination and proteasomal degradation; miR-122-mediated reductions in OGT led to higher RYR1 abundance in BC [152].In addition, Yang et al. found that O-GlcNAcylation of p53 at Ser 149 in MCF-7 cells reduced Thr 155 phosphorylation, rendering p53 partially resistant to ubiquitin-dependent proteolysis under streptozotocin treatment [153], affecting cell viability (Fig. 5). The gene discussed is RYR1; the disease is breast cancer.