The differential binding to dyes like Nile Red could be useful classifiers in lieu of high-resolution structures, as well as in seeding amplifications assays where differences in dye binding have emerged as one of the key distinguishing features of samples isolated from different synucleinopathies [i.e., in the amplification of fibrils from MSA versus PD colony-stimulating factor (CSF) samples (53)]. Here, CSF2 is linked to multiple system atrophy.