Refeeding a fasted animal significantly increases CART immunoreactivity in the NG of lean11 but not HF fed obese animals.60 Post-prandial vagal activation is dependent on the amount of kcal consumed as well as macronutrient makeup, with fat consumption leading to greater activation, for the same amount of kcal, than carbohydrates and proteins.61 Following refeeding, CART+ neurons were present in the NG of all animals, however there were no increase in the number of CART+ neurons in the HF-HF rats compared to the LF groups, despite receiving a fat-rich meal. Here, CARTPT is linked to hydrops fetalis.