To test if the effect of NINJ1 on plasma membrane fragility under mechanical tension is a general, cell-type autonomous phenomenon, we tested the ZOS cells, an osteosarcoma cell line with higher endogenous NINJ1 mRNA and protein levels compared to HeLa cells (Fig. 2d, e, Extended Data Fig. 5), to see if its plasma membrane is more prone to rupture under tension. Here, NINJ1 is linked to osteosarcoma.