It was reported that the PIK3CA‐mutant CRC cell lines are sensitive to glutamine deprivation.[19] However, the knockdown of GLS (GLS‐KD) did not significantly affect the proliferation of both PIK3CA‐wildtype (PIK3CA‐WT, MC38, CT26, and SW480) and PIK3CA‐mutant (HCT116) cells (Figure 1C; Figure S1B, Supporting Information). Here, GLS is linked to colorectal carcinoma.