Wild-type MYH1 and its variants were observed throughout the COS-7 cell body, except for the nucleus (Supplementary Figs. 9 and 10), whereas other members of the myosin superfamily associated with hearing loss, such as MYO3A and MYO15A, have been shown to be localized to the tips of filopodia in COS-7 cells and stereocilia in hair cells63,64. This evidence concerns the gene MYO3A and hearing loss disorder.