Finally, the effect of EC on ferroptosis in lung cancer cells was tested, and the results revealed that EC treatment significantly increased the Fe2+ concentrations in H460 and H1299 cells (Fig 2F) while inhibiting the expression of the ferroptosis-related proteins SLC7A11, GPX4, and FTH1 (Fig 2G). The gene discussed is GPX4; the disease is lung carcinoma.