WT1 and diabetes mellitus: In keeping with our findings on WT1 counts and expression of podocyte proteins, glomerular F-actin content (phalloidin fluorescence intensity) and F-actin colocalization with synaptopodin did not show significant differences among the groups of mice, implying that deletion of iPLA2γ and diabetes did not substantially affect cytoskeleton organization and foot processes (S4 Fig).