Intracellular staining (ICS) revealed significantly higher IFN-γ levels or a greater tendency for production of IFN-γ (Figure 4, D and E, and Supplemental Figure 5F) and TNF-α (Figure 4, F and G, and Supplemental Figure 5G) by T cells, especially in tumor-infiltrating T cells derived from Tmed4ΔTreg mice. Here, TNF is linked to neoplasm.