To identify the potential influence of TMED4 on the regulatory function of Tregs within the tumor microenvironment, we conducted correlation analyses of the gene expression profiles obtained from various cancer datasets including colon adenocarcinoma (COAD), rectal adenocarcinoma (READ), lung adenocarcinoma (LUAD), liver hepatocellular carcinoma (LIHC), and gastric cancers (stomach adenocarcinoma [STAD]) sourced from The Cancer Genome Atlas (TCGA). This evidence concerns the gene TMED4 and rectum adenocarcinoma.