GFAP and craniosynostosis: In the MCAO model, cottonseed oil/CSO treatment significantly decreased the number of C3d/GFAP double-positive cells and upregulated C3d protein expression, increased the number of S100A10/GFAP double-positive cells and downregulated S100A10 protein expression, and inhibited protein expression of TLR4 and NF-κB, which in turn inhibited the release of IL-1β, IL-6 and TNF-α, and ultimately ameliorated blood–brain barrier disruption and neuronal damage.