In addition, the expression of NLRP3, Caspase1, and GSDMD in the cross-sections of the mice aortas was confirmed by IHC (Figure 5F-G), and the release of IL-1β, IL-18, and other two critical AAA-related cytokines (TNF-α and IL-6) in the mice serum was confirmed by ELISA assays (Figure 5H). This evidence concerns the gene GSDMD and triple-A syndrome.