For example, post-QC TOPACIO cluster 0 corresponded to cells with small round nuclei with intense plasma membrane staining for CD4 and nuclear staining for FOXP3, consistent with T regulatory cells (TRegs, Fig. 6e), cells in cluster 21 were high in panCK and γH2AX, indicative of breast cancer cells containing DNA damage (Fig. 6g), and cells in cluster 35 were conventional CD4+ helper T cells (TCons) adjacent to panCK+ tumor cells (captured as a manifestation of spatial crosstalk; Fig. 6h). Here, CD4 is linked to breast cancer.