THY1 and Parkinson disease: To explore the effects of genotype and microbiome on the metabolome in a PD mouse model, “Line 61” Thy1-ASO and wild-type (WT) littermates were reared in specific pathogen-free (SPF; standard laboratory microbiome) or germ-free (GF) conditions to 4 months of age, when ASO mice display robust motor symptoms, constipation-like phenotypes, oxidative stress, and mitochondrial alterations19.