Considering the essential role of IFN-γ in anti-tumor responses43–45, we evaluated the ability of Hif1α–/– T cells to kill tumor cells by co-culturing pre-activate WT or Hif1α–/– hypoxic T cell with MB49 bladder tumor cells at a ratio of 2:1. The gene discussed is HIF1A; the disease is urinary bladder neoplasm.