DIAPH1 and melanoma: Notably, in the relatively fast mesenchymal B16-F1 mouse melanoma cells, which form mostly transient adhesions, active mDia1 and mDia3 are also prominently localized at the cell cortex of the trailing edge, but in this case, their combined loss reduced migration rate by almost 20%, suggesting that this may have been caused by their stronger adhesion compared to Dictyostelium amoeba (18).