To determine whether this reduced replication was due to differences of viral genes transcription after replacing MHV68 M7 with KSHV K8.1, we examined expression levels of viral immediate early (ORF50, ORF57, and ORF71), early (ORF4, ORF6, and ORF9), and late (ORF8, ORF65, and ORF75C) genes after 3, 6, 9, 12, and 15 hours infection. This evidence concerns the gene CTTNBP2 and infection.