STAT3 is palmitoylated at C108, which enhances its recruitment to membranes and promotes Th17 cell differentiation has been reported.[54] Additionally, arginine methylation of STAT3 by PRMT1 facilitates astrocyte differentiation, with R31 identified as a potentially crucial site.[56] A study revealed that EZH2 methylates STAT3 at the K180 site, promoting clonogenic growth in glioblastoma multiforme, characterized by a subpopulation of stem‐like cells.[57] Here, we found that STAT3's K177/K180 and Y293 sites are involved in MVEs translocation for maintaining the pluripotency of mESC. This evidence concerns the gene EZH2 and glioblastoma.