CD38 and B-cell chronic lymphocytic leukemia: In addition to the more progressed leukemic phenotype, most of these CLL cell lines have reduced CD38-expression,33,34,35,36 therefore we utilized the mature B lymphoma-derived cell line, Ramos, expressing high levels of CD3838 and generated knockout variants (Ramos.CD38KO and Ramos.LGALS1KO) using CRISPR-Cas9 (Figures 6A, S11A, and S11B).