We identified enriched upstream regulators from significantly DE genes in response to the 68-1 RhCMV/TB vaccines, including cytokines (e.g., IL-15, IL-2, IL-21, IL-18, and IL-4, IFN gamma) and transcription factors (e.g., STAT1, TNF, TBX21, STAT5, and STAT3). This evidence concerns the gene TNF and tuberculosis.