Using cyst area (mm2) of central OCT cross-sections as a measure of cyst severity, we observed that cysts were nearly completely ameliorated in vector-treated Rs1-KO eyes (n = 10) and hypertonic buffer-injected Rs1-KO eyes, in contrast to untreated eyes at 1 MPI (vector-treated: 0.001871 mm2, p < 0.0001; hypertonic buffer: 0.002878 mm2, p < 0.0001, untreated: 0.01691 mm2, Figures 2A,B). This evidence concerns the gene RS1 and cyst.