To uncover the mechanisms of how exactly hepatocyte E4bp4 deficiency mitigates liver fibrosis, we reasoned that hepatocyte E4BP4 might impact the duration and degree of activation of HSCs, which are the central driver of liver fibrosis.[7] To test this hypothesis, we designed a medium transfer experiment to examine the impact of hepatocyte E4BP4 on the activation of HSCs. This evidence concerns the gene NFIL3 and Hepatic fibrosis.