We used the induction of α‐SMA in liver tissue as a marker of liver fibrosis.[7] As a potent liver fibrosis‐inducing hepatotoxin, carbon tetrachloride (CCl4) has been used to generate chemical‐induced liver fibrosis in mice.[36] Following the 8‐week treatment of CCl4, hepatic E4BP4 protein levels were also increased, in parallel with the markedly elevated α‐SMA, a classical marker of liver fibrosis (Figure1A).[37, 38] Next, we fed WT mice with NASH diet rich in saturated fat, cholesterol, and fructose to induce diet‐induced MASH[21] and examined liver E4BP4 expression during feeding. Here, ACTA1 is linked to metabolic dysfunction-associated steatohepatitis.