We induced iSLK.219 cells with Dox and collected cell lysates at 0h, 24h, 48h, and 72h, representing the latent (0h), early-lytic (24h-48h), and late-lytic (48h-72h) stages of infection, and monitored the levels of BiP by immunoblot throughout a time course of reactivation (Fig 1B). Here, HSPA5 is linked to infection.