Bolis et al. (Bolis et al. 2021) analyzed the differentiation trajectory of PCa using scRNA-seq with the finding that PIK3CA, TP53, FOXA1, KMT2C, and PTEN gene point mutations promote the accumulation of AR signaling, which leads to epithelial cells mutating into primary PCa. This evidence concerns the gene PIK3CA and posterior cortical atrophy.