Hawley et al. (Hawley et al. 2023) performed scRNA-seq analysis on the metastasis sites of mCSPC patients treated with ADT and anti-PD-1 to found that TNF + CD4-Tregs, LAG3 + CD8 + T cells and GITR + Tregs were significantly increased in mCSPC tissues and metastasis tissues (bone, lymph node and liver) after treatment, which indicated that ICIs combined with ADT could change the pattern of TIME in PCa. The gene discussed is CD8A; the disease is posterior cortical atrophy.