The only cancer subtypes that showed significant differences in splicing antigenicity were between SF3B1-wt and mutant samples in BRCA and HNSC (Fig. 4A and B; Supplementary Table S2), with higher splicing antigenicity in mutant samples (BRCA P value = 0.017 and Cohens d = 1.22; HNSC P value = 0.05 and Cohens d = 0.33, Wilcoxon test). The gene discussed is SF3B1; the disease is cancer.