To determine whether MG-specific Nrf2 knockdown resulted in attenuated HO-1 expression in the ischemic brain that subsequently led to exacerbated ischemic brain injury after stroke, we assessed the frequency of Nrf2 and HO-1 expression in MG in male and female Nrf2fl/fl-Cx3cr1CreERT2/+ and control Cx3cr1CreERT2/+ MCAO mice. The gene discussed is HMOX1; the disease is stroke disorder.