Figure 1B shows representative samples of different HLA-G expression levels (negative, low, intermediate, high) at the tumor center and the invasion front. HLA-G showed a similar expression pattern at the tumor center and the invasion front without significant differences in expression levels (Spearman correlation coefficient r=0.86 [95%-CI=0.82-0.90]; Figure 1C). A Shapiro-Wilk test performed on HLA-G distribution indicated that HLA-G protein expression is not normally distributed in our cohort which could be attributed to the size of the cohort (P=0.01, significance level threshold P<0.05). Here, HLA-G is linked to neoplasm.