To explore the effect of APOE4 on neuronal loss and tauopathy, we determined the number of NeuN+ cells and intensity of NeuN, MAP2, phospho-tau (p-S202/T205) (AT8) and phospho-tau (p-T231) (AT180) via immunostaining (Fig. S1i–k). Here, RBFOX3 is linked to tauopathy.