Consequently, exercise‐mediated circFndc3b exerted neuroprotective effects in ischemic stroke by regulating the circFndc3b/ENO1/Klf2 pathway and establishing a positive feedback loop via circFndc3b/ENO1/FUS, making circFndc3b a potential therapeutic target for stroke intervention. This evidence concerns the gene KLF2 and ischemic stroke.