Using western blotting, we compared the protein expression of MCU, MICU1, and MICU2 in a non-tumor colorectal epithelial cell line, NCM356, and in 5 CRC cell lines (HT29, DLD1, SW480, HCT116, and SW620) with different mutation and aggressiveness profiles. The gene discussed is MCU; the disease is colorectal carcinoma.