After that, we found that 6 immune checkpoints (CD274, CTLA4, LAG3, LGALS9, PDCD1, and PDCD1LG2) were significantly upregulated in the high-glioma-risk group, while RDH5, RDH10 and DHRS3 simultaneously have a substantial positive connection with PDCD1LG2. The gene discussed is CD274; the disease is central nervous system cancer.