To validate ALKBH5’s role in AML resistance to ADR, si-ALKBH5-1, si-ALKBH5-2, and si-ALKBH5-3 were transfected into HL60/ADR and KG-1/ADR cells to suppress ALKBH5 expression (P < 0.05, Figure 2A). This evidence concerns the gene ALKBH5 and acute myeloid leukemia.