We evaluated key hub miRNA identified in the familial cohort for its potential to serve as a biomarker for T1D by validating it in an independent cohort of sporadic T1D cases by way of selecting the highest-order network motif consisting of hsa-miR-320a-3p, with MYC and FOXO1 as the key transcription factors regulating the expression of key miRNA target genes such as PTEN, BCL2, and AKT1. We also provided additional evidence for the involvement of their key interacting partners such as BLIMP1, GSK3B, CAV1, IL10 and TGFB in T1D pathogenesis. Here, PTEN is linked to type 1 diabetes mellitus.