Groups vaccinated with adsorbed/non-adsorbed L1V and adsorbed B5V had minimal weight loss and high survival rates (80-100%). Other groups, like adsorbed A33V (100% survival), non-adsorbed A33V (80%), and non-adsorbed B5V (60%), lost more weight (about 20%). A27V formulations elicited strong antibody responses but failed to protect against virus infection effectively, mirroring results with the histidine-tagged VACV A27 protein. Here, IGKV3-20 is linked to viral infectious disease.