In our study, the ROS1 mutation rate was 1.0% (16/1586), in single organ metastasis, ROS1-positive NSCLC was more transferred to the pleura (37.5% vs.16.8%, P = 0.044) and lung (31.3% vs.12.4%, P = 0.045) compared with wild type. Here, ROS1 is linked to non-small cell lung carcinoma.