NFKB1 and fungal infectious disease: Remarkably, hym, def1 and cru were not induced by fungal challenge (Fig. 2A; M. brunneum vs sham: | z | <1.061, p > 0.288 for all comparisons), and def2 was significantly downregulated by spore injection (z = 3.80; p < 0.0001), suggesting that L. niger NF-κB pathways do not respond to systemic fungal infections by producing AMPs.