In summary, the results found in this study are of great significance, as it was possible to prove the reliability of the combination of tests used in serological and molecular screening, mainly HBV NAT and anti-HBc, performed in Brazilian blood centers, with the verification of the impact of different profiles found in blood donations and the confirmation of results with viral load detection in a reference center for hepatitis in the Brazilian Amazon. This evidence concerns the gene KRT88P and Hepatitis.