Combining on-chip continuous flow mixing with immunomagnetic isolation, an in situ Raman assay was developed to capture exosomes using anti-CD63 conjugated magnetic nanoparticles in microchannels with staggered triangular pillar array; the enriched exosomes were magnetically fixed on the Raman detection segment, and EpCAM-functionalized Raman beads with high nitrile densities were used as the probe to quantitatively detect exosomes from PCa cells (LNCaP) and healthy prostate cells (PrEC) by monitoring the intensity of the SERS peak [254]. Here, EPCAM is linked to posterior cortical atrophy.